Good afternoon everyone, I just made a new HISAT2 index for the newest Bos taurus genome, ARS-UCD1.2.
Link to source genome:
https://ftp.ncbi.nlm.nih.gov/genomes/refseq/vertebrate_mammalian/Bos_taurus/latest_assembly_versions/GCF_002263795.1_ARS-UCD1.2/GCF_002263795.1_ARS-UCD1.2_genomic.fna.gz
Path to fasta file:
/shared/projects/theileria_host_pathogen/Genomes/Bos_taurus.ARS-UCD1.2.fna
Path to index files (.ht2):
/shared/projects/theileria_host_pathogen/Genomes/
I created them with the commands (as found in /shared/projects/theileria_host_pathogen/MrIndex.sh):
module load hisat2
hisat2 --version
hisat2-build -p 32 /shared/projects/theileria_host_pathogen/Bos_taurus.ARS-UCD1.2_genomic.fna /shared/projects/theileria_host_pathogen/Bos_taurus.ARS-UCD1.2_genomic.fai
Note: I renamed the files after unzipping and after index creation.