# Problem to use the Busco tool with odb12 for Eukaryotes lineage

**URL:** <https://community.france-bioinformatique.fr/t/problem-to-use-the-busco-tool-with-odb12-for-eukaryotes-lineage/6836>\
**Category:** Galaxy\
**Created:** [Mai 5, 2026, 7:46 UTC](https://community.france-bioinformatique.fr/t/problem-to-use-the-busco-tool-with-odb12-for-eukaryotes-lineage/6836 "2026-05-05T19:46:01Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![Silke\_Jensen](https://community.france-bioinformatique.fr/user_avatar/community.france-bioinformatique.fr/silke_jensen/32/608_2.png) [@Silke\_Jensen](https://community.france-bioinformatique.fr/u/Silke_Jensen)\
**Post date:** [Mai 5, 2026, 7:46 UTC](https://community.france-bioinformatique.fr/t/problem-to-use-the-busco-tool-with-odb12-for-eukaryotes-lineage/6836/1 "2026-05-05T19:46:01Z")

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Hi,

I wanted to use the odb12 database for the Eukaryotes lineage for Busco analyses of my genome assemblies (tool **Busco** Assess genome assembly and annotation completeness(Galaxy Version 5.8.0+galaxy2)). I didn't manage to do this by chosing "All odb12 2026-03-20-145944" in "Cached database with lineage" and "Eukaryota" in "Lineage". I didn't find any other possiblity to do it since there were no choices available in "Select lineage". The Busco failed when I made this odb12 and Eukayotes choice with the error message "2026-05-05 20:56:18 ERROR: Unable to run BUSCO in offline mode. Dataset /cvmfs/data.galaxyproject.org/byhand/busco/all-odb12-2026-03-20-145944/lineages/eukaryota\_odb10 does not exist."

Is there a way to solve this problem? Thank you in advance for your help.