Issue downloading SRA files from NCBI


In the context of my PhD project, I'm working with publicly available scRNA-seq datasets. I need to download SRA files from NCBI in order to extract the fastq files and process them. I use the wget command to do this:


However, whenever I try to dowload a file specifically from NCBI it is rejected with a 403 forbidden error.

--2022-03-25 08:55:20--
Connecting to||:443... connected.
HTTP request sent, awaiting response... 403 Forbidden
2022-03-25 08:55:21 ERROR 403: Forbidden.

Are we not allowed to download from NCBI using the IFB cluster? Would it be possible to take a look at this because so far I've been unable to download the files that I need?

Thank you!


Bonjour Jessica,
It seems that your url is not accessible.
If you search for the run SRR8262952, you will find the page :
In the tab "data access", we find the url allowing to access the data:
I hope this will help you.


I apologise for the late reply!
Thank you for your feedback, yes it seems I was using an older URL that is no longer available. I'll have to be more careful about that in the future.