I have tried to align sequnces to Silva several times and now with GreenGens,on Meatagenomics/ mothur pipeline, but it still don't working,
can you tell me please what have I to do? I used primers for the V3/V4 region of the rRNA 16S.
Here's the error message "This job was terminated because it used more memory than it was allocated." and it was impossible to report the error, i've got this message "An error occurred sending the report by email: Mail is not configured for this Galaxy instance"
Any help would be greatly appreciated!
It seems that you are working on a Galaxy server.
I didn't know that the Galaxy server of the IFB was in production.
Obviously it's a ressources problem, that you can't modify as a user.
Normally on galaxy when you have a "red dataset", you can click on the bug symbol to directlty contact the support team of the Galaxy, who will have access to your history and so check if there is an other problem with your data.
Did you test this solution ?
Hello Maria,
the Galaxy server of the IFB was in production, but once I clicked on the bug symbol to contact the support team, this message "An error occurred sending the report by email: Mail is not configured for this Galaxy instance" appears.
I have to thank for your help in your previous email. I tried FROGS and it works well , but I'm trying to work on mothur too, that's why I find this issue on alignment with bank datasets.
In my work, I have a part where I have to use two tools at least to make clusters on my data
Have a nice day too
Sorry for being unclear, I was talking about the "Align.seqs" tool, my inputs is the fasta file from "Unique.seq" and for the reference, I tried Silva and then GreenGens.
thanks for your recommandation, indeed my sequences come from Illumina Miseq
, but I'm trying to work on mothur too, that's why I find this issue on alignment with bank datasets.
