Sorry for being unclear, I was talking about the "Align.seqs" tool, my inputs is the fasta file from "Unique.seq" and for the reference, I tried Silva and then GreenGens.
thanks for your recommandation, indeed my sequences come from Illumina Miseq
Sorry for being unclear, I was talking about the "Align.seqs" tool, my inputs is the fasta file from "Unique.seq" and for the reference, I tried Silva and then GreenGens.
thanks for your recommandation, indeed my sequences come from Illumina Miseq