RNA star error file does contain alignment data

Galaxy
1

Hello,
When I run RNA STAR for mapping by using "Trimmomatic on collection data". Some errors happened. Like:

1
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I already uploaded the reference genome fasta file and .gff file for analysis.
Could you give me some suggestions regarding the error?
Thanks a lot!
Have a good day!
Best regards,
Zibo

2

Dear Ribo,

I just gave a try on Trimmomatic/STAR on a dataset collection (as paired-end) and it worked just fine. Have you checked that trimmomatic worked well or checked RNA STAR logs?
I tried with data mapped on GRCh38 (built-in) and a custom gtf file.

Best,

Stephanie

3

Hi Stephanie,

Thanks for your reply!

Yes, it works now!

Have a good afternoon!

Best regards,

Zibo